E4884

Ethylenediaminetetraacetic acid disodium salt dihydrate

ACS reagent, 99.0-101.0%

• Synonym(s): Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na₂, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na₂
• Empirical Formula (Hill Notation): C₁₀H₁₄N₂Na₂O₈ · 2H₂O
• CAS Number: 6381-92-6
• Molecular Weight: 372.24
• Beilstein: 3900609
• EC Number: 205-358-3
• PubChem Substance ID: 24894586
• NACRES: NA.77

Properties

• grade: ACS reagent
• Quality Level: 200
• Assay: 99.0-101.0%
• form: solid
• impurities: ≤0.005% Insoluble matter; ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)
• pH: 4.0-6.0 (25 °C, 5%)
• mp: 248 °C (dec.) (lit.)
• solubility: H₂O: 100 g/L at 20 °C
• cation traces: Fe: ≤0.01%; heavy metals: ≤0.005% (by ICP-OES)
• storage temp.: room temp
• SMILES string: O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
• InChI: 1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
• InChI key: OVBJJZOQPCKUOR-UHFFFAOYSA-L

Description

General description

EDTA is a chelator of divalent cations. It inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Application

Ethylenediaminetetraacetic acid disodium salt dehydrate has been used as a component:

• of radioimmunoprecipitation assay buffer (RIPA buffer) for preparation of C2C12 mouse skeletal myoblasts whole cell lysate
• of lysis buffer and IgG resin wash and equilibration buffer for the purification of human proteasome regulatory particles (RPs)
• of M-buffer for the isolation of human cardiomyocyte progenitor cells (CMPCs) from fetal heart

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, majorly used in preventing clotting of blood. EDTA may trigger aggregation of platelets resulting in pseudothrombocytopenia. EDTA along with tetracycline serves as a root conditioning agent and provides relief in gum infection. EDTA-tetracycline therapy decreases coronary artery calcium levels in atherosclerosis. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It also enhances the antimicrobial functionality of lysozyme and improves shelf life of cheese.

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Warning
• Hazard Statements: H332 - H373
• Precautionary Statements: P260 - P271 - P304 + P340 + P312 - P314 - P501
• Hazard Classifications: Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
• Target Organs: Respiratory Tract
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves