P0290

Phenol red solution

liquid, 0.5%, sterile-filtered, BioReagent, suitable for cell culture

• Synonym(s): Phenolsulfonphthalein solution
• CAS Number: 143-74-8
• MDL number: MFCD00003552
• UNSPSC Code: 12352207
• PubChem Substance ID: 24898090
• NACRES: NA.75

Properties

• sterility: sterile-filtered
• Quality Level: 200
• product line: BioReagent
• form: liquid
• concentration: 0.5%
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• SMILES string: O=S1(C2=CC=CC=C2C(C3=CC=C(O)C=C3)(C4=CC=C(O)C=C4)O1)=O
• InChI: 1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H
• InChI key: BELBBZDIHDAJOR-UHFFFAOYSA-N

Description

General description

Phenol red belongs to the class of triphenylmethane dyes and is widely used in the textile industry.[1]

Application

Phenol red solution has been used:

• as a tracer dye for microinjection of zebrafish eggs[2]
• as a component of the mMW medium for the preparation of mouse embryos[3]
• in the preparation of versene-ethylenediamine tetraacetic acid (EDTA) solution[4]
• as a pH indicator in Dulbecco′s modified Eagle medium (DMEM) during glycolytic stress (GST) tests and mitochondrial stress tests (MST) to evaluate metabolic function in cells.[5]

Biochem/physiol Actions

Phenol red solution acts as a pH indicator for the culture medium.[6]Phenol red solution acts as a pH indicator for the culture medium that turns yellow at a pH of 6.4 or lower and changes into red at a pH of 8.2 or higher. It enables a fast and simple assessment of the culture′s health. In addition to its application as a pH indicator in culture mediums, Phenol red is used to detect human peroxidase enzyme activity, as well as cellular hydrogen peroxide levels, in various experiments.

Features and Benefits

High-quality, endotoxin-tested solution, suitable for mammalian cell culture.

Preparation Note

Prepared in Dulbecco′s Phosphate Buffered Saline.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable