Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.
Contains 1 mg o-phthaldialdehyde (P0657) per mL solution with 2-mercaptoethanol as the sulfhydryl moiety.