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51456

Sigma-Aldrich

Urea

BioUltra, for molecular biology, ≥99% (T)

Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

assay

≥99% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.1% biuret
<0.005% formaldehyde (HCHO)

ign. residue

≤0.01% (as SO4)

loss

≤0.1% loss on drying, 20 °C (HV)

pH

8.0-10.0 (20 °C, 8 M in H2O)

mp

132-135 °C (lit.)

solubility

H2O: 8 M at 20 °C, clear, colorless

density

1.335 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤10 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

SMILES string

NC(N)=O

λ

8 M in H2O

UV absorption

λ: 260 nm Amax: ≤0.03
λ: 280 nm Amax: ≤0.02

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Other Notes

Denaturing reagent; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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