PIPES

Name: PIPES
Brand: SIGMA

BioXtra, Molecular Biology, ≥99.5% (T)

Synonym(s):

1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):

C₈H₁₈N₂O₆S₂

CAS Number:

5625-37-6

Molecular Weight:

302.37

Beilstein:

817713

EC Number:

227-057-6

MDL number:

MFCD00006159

UNSPSC Code:

12161700

PubChem Substance ID:

57652837

NACRES:

NA.25

Key Documents

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grade

Molecular Biology

Quality Level

100

product line

BioXtra

Assay

≥99.5% (T)

form

powder

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

ign. residue

≤0.2% (as SO₄)

loss

≤0.2% loss on drying, 110 °C

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

anion traces

chloride (Cl^-): ≤200 mg/kg
sulfate (SO₄^2-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤100 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

0.1 M in 1 M NaOH

UV absorption

λ: 260 nm A_max: 0.055
λ: 280 nm A_max: 0.040

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

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Related Categories

Biochemicals

Biological Buffers

Chemistry & Biochemicals

High-Purity Biochemicals and Reagents

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Show Differences

1 of 4

This Item	P1851	P8203	P6757
Sigma-Aldrich 80635 PIPES	Sigma-Aldrich P1851 PIPES	Sigma-Aldrich P8203 PIPES	Sigma-Aldrich P6757 PIPES
assay ≥99.5% (T)	assay -	assay ≥99% (titration)	assay ≥99% (titration)
grade for molecular biology	grade BioPerformance Certified, for molecular biology	grade -	grade -
product line BioXtra	product line -	product line BioXtra	product line -
Quality Level 100	Quality Level 400	Quality Level 400	Quality Level 400
form powder	form crystalline powder	form powder	form crystalline powder
solubility 0.1 M NaOH: 0.1 M at 20 °C, clear, colorless	solubility 1 M NaOH: 20 + 80 mL g, clear, colorless, soluble	solubility 1 M NaOH: 0.5 M at 20 °C, clear, colorless	solubility 1 M NaOH: 20 + 80 mL g, clear, colorless

Application

Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics.: The use of PIPES buffer in this study was integral to maintaining the stability of immunoglobulin G during differential scanning fluorimetry. This method is crucial for evaluating the stability of antibody-based therapeutics (Moggridge et al., 2017).

Other Notes

Hybrid selection of mRNA - component of prehybridization buffer.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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R. Jagus

Methods in Enzymology, 152, 570-570 (1987)

Hydrogen sulfide deactivates common nitrobenzofurazan-based fluorescent thiol labeling reagents.

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Sulfhydryl-containing compounds, including thiols and hydrogen sulfide (H2S), play important but differential roles in biological structure and function. One major challenge in separating the biological roles of thiols and H2S is developing tools to effectively separate the reactivity of these

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Veronique Adoue et al.

Molecular systems biology, 10, 754-754 (2014-10-19)

Most complex disease-associated genetic variants are located in non-coding regions and are therefore thought to be regulatory in nature. Association mapping of differential allelic expression (AE) is a powerful method to identify SNPs with direct cis-regulatory impact (cis-rSNPs). We used

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The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(32), 10780-10792 (2014-08-08)

Repetitive transcranial magnetic stimulation (rTMS) is increasingly used as a treatment for neurological and psychiatric disorders. Although the induced field is focused on a target region during rTMS, adjacent areas also receive stimulation at a lower intensity and the contribution

Interrogation of allelic chromatin states in human cells by high-density ChIP-genotyping.

Nicholas Light et al.

Epigenetics, 9(9), 1238-1251 (2014-07-24)

Allele-specific (AS) assessment of chromatin has the potential to elucidate specific cis-regulatory mechanisms, which are predicted to underlie the majority of the known genetic associations to complex disease. However, development of chromatin landscapes at allelic resolution has been challenging since

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