While stability studies at 37°C have not been conducted, this product ships at ambient temperature and is fairly robust in the powder form. Solutions tend to degrade and lose activity fairly rapidly in the presence of oxygen and at alkaline pH. Additional information is available in the product datasheet available for L-Ascorbic Acid, product A7506:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/221/043/a7506pis.pdf
A4403
L-Ascorbic acid
powder, suitable for cell culture, γ-irradiated
Synonym(s):
L-Threoascorbic acid, Antiscorbutic factor, Vitamin C
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About This Item
Empirical Formula (Hill Notation):
C6H8O6
CAS Number:
Molecular Weight:
176.12
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
EC Number:
200-066-2
Beilstein/REAXYS Number:
84272
MDL number:
Form:
powder
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InChI key
CIWBSHSKHKDKBQ-JLAZNSOCSA-N
InChI
1S/C6H8O6/c7-1-2(8)5-3(9)4(10)6(11)12-5/h2,5,7-10H,1H2/t2-,5+/m0/s1
SMILES string
OC([C@]([C@@H](O)CO)([H])O1)=C(O)C1=O
biological source
synthetic (organic)
sterility
γ-irradiated
form
powder
mol wt
Mw 176.12 g/mol
technique(s)
cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable
color
white to off-white
pH
1.0-2.5 (25 °C, 176 g/L in water)
mp
190-194 °C (dec.)
solubility
H2O: 10 mg/mL
Quality Level
Gene Information
human ... SLC23A2(9962)
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This Item | 795437 | A2218 | 47863 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form powder | form crystals or granules, powder or chunks | form solid | form solid |
| solubility H2O: 10 mg/mL | solubility water: soluble 176 g/L at 20 °C | solubility water: soluble 176 g/L at 20 °C | solubility water: soluble 176 g/L at 20 °C |
| pH 1.0-2.5 (25 °C, 176 g/L in water) | pH 1.0-2.5 (25 °C, 176 g/L in water) | pH 1.0-2.5 (25 °C, 176 g/L in water) | pH 1.0-2.5 (25 °C, 176 g/L in water) |
| mp 190-194 °C (dec.) | mp 190-194 °C (dec.) | mp 190-194 °C (dec.) | mp 190-194 °C (dec.) |
| biological source synthetic (organic) | biological source synthetic (organic) | biological source synthetic | biological source - |
Application
For use in cell culture applications as an antioxidant and to regenerate tocopherol.
L-ascorbic acid has been used:
L-ascorbic acid has been used:
Biochem/physiol Actions
L-ascorbic acid mainly exhibits antioxidant properties. It protects plants from oxidative stress and mammals from diseases associated with oxidative stress.[4] L-ascorbic acid mainly protects from hydroxyl radicals, superoxide and singlet oxygen. In addition, it also reduces the membrane-linked antioxidant α-tocopherol (oxidised form).[5] L-Ascorbic acid enhances endothelium-dependent vasodilation in various disorders, including diabetes, coronary artery disease, hypertension and chronic heart failure.[6]
General description
L-ascorbic acid is an unsaturated γ-lactone ring containing an enediol configuration (–COH=COH) attached with a carbonyl group.[6]
related product
Product No.
Description
Pricing
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
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Xi Wang et al.
Archives of oral biology, 57(9), 1231-1240 (2012-03-30)
This study focused on the characterization of stem cells from human exfoliated deciduous teeth (SHED) in comparison with dental pulp stem cells (DPSCs) to certify SHED as a key element in tissue engineering. In the present study, SHED and DPSCs
P L Conklin et al.
Plant physiology, 115(3), 1277-1285 (1997-12-09)
The biosynthesis of L-ascorbic acid (vitamin C) is not well understood in plants. The ozone-sensitive Arabidopsis thaliana mutant vitamin c-1 (vtc1; formerly known as soz1) is deficient in ascorbic acid, accumulating approximately 30% of wild-type levels. This deficiency could result
R Heller et al.
The Journal of biological chemistry, 274(12), 8254-8260 (1999-03-13)
Ascorbic acid has been shown to enhance impaired endothelium-dependent vasodilation in patients with atherosclerosis by a mechanism that is thought to involve protection of nitric oxide (NO) from inactivation by free oxygen radicals. The present study in human endothelial cells
Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing.
Davey MW
Journal of the Science of Food and Agriculture, 80, 825-860 (2000)
Ying Li et al.
Protein & cell, 7(7), 478-488 (2016-06-09)
Werner syndrome (WS) is a premature aging disorder that mainly affects tissues derived from mesoderm. We have recently developed a novel human WS model using WRN-deficient human mesenchymal stem cells (MSCs). This model recapitulates many phenotypic features of WS. Based
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Do you happen to have any information on how long L-ascorbic acid remains stable at 37°C? Do you have any idea after how much time the full activity of this compound is lost?
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What is the right storage condition for the powder upon its arrival?
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This product may be stored at room temperature in powder form.
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What is the recommended storage method for stock solutions of item A4403, L-Ascorbic acid?
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Stock solutions of item A4403, L-Ascorbic acid, can be stored at -20°C for long-term (months to years) stability.
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In what solvents is A4403 soluble?
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The product A4403 is soluble in water at a concentration of 10 mg/ml, resulting in a clear solution.
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