MilliporeSigma
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A7811

Sigma-Aldrich

Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse

clone EA-53, ascites fluid

Synonym(s):
Anti-1110008F24Rik, Anti-MGC107582, Actinin Antibody, Anti-ACTN2, Sarcomeric Alpha Actinin Antibody, Anti-Actinin α2, Anti-CMD1AA, Sarcomeric Alpha Actinin Antibody - Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

EA-53, monoclonal

mol wt

antigen 100 kDa

contains

15 mM sodium azide

species reactivity

fish, snake, frog, goat, hamster, pig, canine, mouse, feline, chicken, lizard, bovine, human, sheep, rat, rabbit

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using human skeletal and cardiac muscle
indirect ELISA: suitable using human and animal muscle tissue
indirect immunofluorescence: suitable using human and animal cultured muscle cells
western blot: 1:2,500 using rat leg muscle

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245)

General description

α-Actinin (ACTN2) is a 100kDa actin-binding protein that is found in muscle as well as non-muscle cells. In smooth muscles, α-actinin is present in dense bodies and plaques whereas in normal skeletal muscles, it is associated with z-discs that define muscle sarcomeres. ACTN2 is located on human chromosome lq42-q43. α-actinin has rod shaped N-terminal domain.
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue.
The antibody is specific for a-skeletal and a-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue. α-actinin is detected predominantly in dense bodies and plaques which are characteristic of that tissue. Immunofluorescent labeling of a large variety of cells with anti a -actinin reveals an extensive association of the proteins with the actin containing stress fibers and, in particular, with their membrane-bound termini.
Monoclonal Anti-α-Actinin (Sarcomeric) (mouse IgG1 isotype) is derived from the EA-53 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rabbit skeletal a-actinin.1 The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2. Monoclonal Anti-α-Actinin (Sarcomeric) may be used for the localization of sarcomeric α-actinin using various immunochemical assays such as ELISA, dot blot, immunoblot, immunohistochemistry, and immunocytochemistry. The antibody is useful in the immunolocalization of α-actinin in normal and neoplastic cultured cells and tissues, and for studies on the state of sarcomeric muscle organization, in normal and pathological situations.

Specificity

The antibody is specific for a-skeletal and α-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).

Immunogen

Rabbit skeletal α-actinin

Application

Cardiac myocytes were stained with monoclonal mouse anti-actinin to visualize the Z-lines after fixation in pre-cooled methanol/acetone mix (1:1) for 10 minutes at 4 °C.
Mouse monoclonal anti-α-actinin (sarcomeric) antibody is suitable for immunohistochemistry (1:800), ELISA, immunofluorescence and western blot (1:2,500) applications.

Biochem/physiol Actions

α-actinin functions as an actin crosslinker and promotes cell migration. Mutations in α-actinin gene locus is observed in patients with heterogeneous hypertrophic cardiomyopathy and in juvenile onset atrial fibrillation.

Physical form

Supplied as ascites fluid with 15 mM sodium azide as a preservative

Storage and Stability

Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots at -20 °C. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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