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A8200

Sigma-Aldrich

Aminopeptidase from Aeromonas proteolytica

lyophilized powder, 50-150 units/mg protein

Synonym(s):

AAP, Aminopeptidase from Vibrio proteolyticus, bacterial leucyl aminopeptidase

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100 UNITS
$536.00
250 UNITS
$1,190.00

$536.00

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100 UNITS
$536.00
250 UNITS
$1,190.00

About This Item

CAS Number:
37288-67-8
EC Number:
3.4.11.10 (BRENDA, IUBMB)
EC Number:
232-874-6
MDL number:
MFCD00166325
UNSPSC Code:
12352204
NACRES:
NA.54

$536.00

Please contact Customer Service for Availability

grade

Proteomics Grade

Quality Level

300

form

lyophilized powder

specific activity

50-150 units/mg protein

mol wt

29.5 kDa

composition

Protein, ~40% biuret

solubility

H2O: soluble 0.9-1.1 mg/mL, clear, colorless

foreign activity

endopeptidase, essentially free

storage temp.

−20°C

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Aminopeptidase from Aeromonas proteolytica lyophilized powder, 50-150 units/mg protein

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P5380

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specific activity

50-150 units/mg protein

specific activity

≥5.0 units/mg protein

specific activity

≥200 units/mg protein

specific activity

7-15 units/mg solid

grade

Proteomics Grade

grade

-

grade

-

grade

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

H2O: soluble 0.9-1.1 mg/mL, clear, colorless

solubility

-

solubility

-

solubility

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear, H2O: soluble, colorless

mol wt

29.5 kDa

mol wt

24.072 kDa by amino acid sequence, 28 kDa by SDS-PAGE

mol wt

21 kDa by gel filtration, 33 kDa by SDS-PAGE

mol wt

27 kDa

General description

A zinc-containing enzyme.

Specificity

Catalyzes the release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Application

Aminopeptidases are a family of widely distributed proteases, which may be used to study many significant biological processes such as protein maturation, hormone production, and peptide digestion. The enzyme has been used to measure the kinetic rate constant for the binding of bestatin, a general protease inhibitor, to aminopeptidase.[1]

Biochem/physiol Actions

Aminopeptidase from Aeromonas proteolytica is a metalloenzyme, which contains 2 atoms of Zn2+ in a single polypeptide with an approximate molecular weight of 29.5 kDa as determined by sedimentation. This enzyme has a high degree of stability, being stable even at temperatures of 70 °C for several hours. Partial inactivation occurs in 8 M urea. Maximum stability and activity are between pH 8.0-8.5. Aminopeptidase from Aeromonas proteolytica can function as an esterase.[2]
Aminopeptidase from Aeromonas proteolytica is involved in protein maturation, hormone production and peptide digestion.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-leucine p-nitroanilide to L-leucine and p-nitroaniline per min at pH 8.0 at 25 °C.

Physical form

Lyophilized powder containing tricine buffer, pH 8.0, zinc chloride and stabilizer.

Preparation Note

Dissolves in water at 0.9-1.1 mg/mL concentration to form a clear, colorless solution.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Precautionary Statements

P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000173550
0000397458
0000127438
0000173550
0000127438

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