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B7435

Sigma-Aldrich

CelLytic B Cell Lysis Reagent

For bacterial cell lysis, standard strength

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This Item
C3228B7310C2360
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

CelLytic B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.

Features and Benefits

  • Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
  • Scalable for 1 to 25 grams of bacterial cell paste
  • No interference with downstream applications such as affinity chromatography, IP, and Western blotting
  • Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Other Notes

Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).

Legal Information

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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