C1261

Carboxypeptidase A−Agarose

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

• Synonym(s): Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase
• Enzyme Commission number: 3.4.17.1 (BRENDA, IUBMB)
• MDL number: MFCD00130718
• NACRES: NA.54

Properties

• biological source: enzyme from bovine pancreas
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: ≥6 units/mL packed gel, 25 °C
• mol wt: ~35,250
• matrix: beaded agarose
• storage temp.: 2-8°C

Description

General description

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Biochem/physiol Actions

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.

Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

Unit Definition

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

Physical form

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

Safety Information

• Pictograms: GHS07,GHS08
• Signal Word: Danger
• Hazard Statements: H315 - H319 - H334 - H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable