C6423

α-Chymotrysin Protease

Cleaves at aromatic residues, suitable for mass spectrometry, from bovine pancreas

• CAS Number: 9004-07-3
• EC Number: 3.4.21.1 (BRENDA, IUBMB)
• EC Number: 232-671-2
• MDL number: MFCD00130481
• UNSPSC Code: 12352204
• eCl@ss: 42010112
• NACRES: NA.26

Properties

• Product Name: α-Chymotrypsin from bovine pancreas, suitable for protein sequencing, salt-free, lyophilized powder
• grade: Proteomics Grade
• Quality Level: 200
• form: salt-free, lyophilized powder
• mol wt: 25 kDa
• suitability: suitable for protein sequencing
• UniProt accession no.: P00767
• storage temp.: 2-8°C
• Gene Information: cow ... CTRB1(618826)

Description

General description

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein.[1] The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.[2]

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry.[3] It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.[3]

Application

α-Chymotrypsin from bovine pancreas is used for the following applications:

• Protein Identification by mass spectrometry (MS)[4]
• The isolation and characterization of myosin heavy chains[5]
• Toxin preparation[6]
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE[7]

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.[8]

Biochem/physiol Actions

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side.[9] α-Chymotrypsin is inhibited by Cu²⁺ and Hg.[9]

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H315,H319,H334,H335,H400
• Precautionary Statements: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves