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Carboxypeptidase B from porcine pancreas

lyophilized powder

Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
PubChem Substance ID:

biological source

Porcine pancreas

Quality Level


lyophilized powder

specific activity

≥125 units/mg protein

mol wt

34,000 Da± 600

purified by

affinity chromatography


Protein, 40-70%

foreign activity

carboxypeptidase A ≤1%
chymotrypsin ≤0.1%
trypsin ≤5%

storage temp.




InChI key


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Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format.[1] It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.[2]


1, 5 mg in glass bottle
Package size based on protein content

Biochem/physiol Actions

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.[3]

Unit Definition

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

Physical form

Contains HEPES buffer salts, zinc chloride and carbohydrate

Preparation Note

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Analysis Note

Protein determined by biuret.


Exclamation markHealth hazard

Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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Technical Information

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