This product contains HEPES buffer salts and a stabilizing carbohydrate component. The material may be reconstituted in water at 1mg/mL. Solution stability studies have not been performed, however, the stock solution is expected to be stable for 1 - 2 months when aliquoted and stored at −20°C. Avoid repeated freeze/thaw cycles. A datasheet is not available for this product. Please see the link below to review the enzyme assay protocol:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/198/734/c9584enz.pdf
C9584
Carboxypeptidase B from porcine pancreas
lyophilized powder
Synonym(s):
Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
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biological source
Porcine pancreas
Quality Level
grade
Proteomics Grade
form
lyophilized powder
specific activity
≥125 units/mg protein
mol wt
34,000 Da± 600
purified by
affinity chromatography
composition
Protein, 40-70%
foreign activity
carboxypeptidase A ≤1%
chymotrypsin ≤0.1%
trypsin ≤5%
storage temp.
−20°C
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
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This Item | C9268 | C9658 | 217356 |
|---|---|---|---|
| grade Proteomics Grade | grade Proteomics Grade | grade - | grade - |
| specific activity ≥125 units/mg protein | specific activity ≥50 units/mg protein | specific activity ≥3 units/mg protein | specific activity ≥100 units/mg protein |
| biological source Porcine pancreas | biological source - | biological source - | biological source Porcine pancreas |
| form lyophilized powder | form ready-to-use solution | form lyophilized powder | form lyophilized |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 34,000 Da± 600 | mol wt ~35 kDa | mol wt homodimer ~90 kDa | mol wt - |
Application
Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.
The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format.[1] It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.[2]
Biochem/physiol Actions
Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.[3]
Packaging
Package size based on protein content
Unit Definition
One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.
Physical form
Contains HEPES buffer salts, zinc chloride and carbohydrate
Preparation Note
Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.
Analysis Note
Protein determined by biuret.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Chciałam zapytać jak przygotować roztwór Carboxypeptidase B from porcine, który występuje w postaci liofilizowanego proszku? Jest dostępna instrukcja?
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Can Product C9584, Carboxypeptidase B from porcine pancreas be used to remove basic C-terminal residues of tryptic peptides?
1 answer-
The use of Product C9584, Carboxypeptidase B from porcine pancreas to remove basic C-terminal residues of tryptic peptides has been described in the literature. Please see the following as an example:The Protein Protocols Handbook, J.M. Walker, Ed., pp. 569, Humana Press, Totawa NJ (1996)
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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