- CB_85011417
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biological source
mouse skin
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
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This Item | 19-147 | CB_93022518 | CB_89030317 |
|---|---|---|---|
| biological source mouse skin | biological source - | biological source Mouse x Mouse Hybridoma | biological source hamster ovary |
| growth mode Suspension | growth mode - | growth mode - | growth mode Adherent |
| karyotype Suspension | karyotype - | karyotype - | karyotype Adherent |
| morphology Suspension | morphology - | morphology - | morphology Adherent |
| products Suspension | products - | products - | products Adherent |
| receptors Suspension | receptors - | receptors - | receptors Adherent |
Cell Line Origin
Mouse myeloma
Cell Line Description
Derivative of P3X63Ag8, known as the Kearney line, the cell line is a non secretor. HGPRT- and HAT sensitive. It is useful for fusion with spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas.
Application
Fusion partner, hybridisation and tumourigenicity studies
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Rapid cell death occurs when cell concentration exceeds 1,000,000 cells/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificate of Analysis
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Certificate of Origin
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