- CB_85011425
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L929 Cell Line from mouse
85011425, mouse C3H/An connective tisue
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Synonym(s):
Earles′s Cells, L Cells, NCTC clone 929 Cells
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biological source
mouse adipose tissue
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
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This Item | CB_08100805 | CB_94082292 | CB_87022501 |
|---|---|---|---|
| biological source mouse adipose tissue | biological source mouse nerve | biological source human blood | biological source mouse skin (fibroblast) |
| growth mode Adherent | growth mode Adherent | growth mode Suspension | growth mode Adherent |
| karyotype Adherent | karyotype Adherent | karyotype Suspension | karyotype Adherent |
| morphology Adherent | morphology Adherent | morphology Suspension | morphology Adherent |
| products Adherent | products Adherent | products Suspension | products Adherent |
| receptors Adherent | receptors Adherent | receptors Suspension | receptors Adherent |
Cell Line Origin
Mouse C3H/An connective tissue
Cell Line Description
Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.
Application
Virus studies: PRV, VSV (Indiana), HSV
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificate of Analysis
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Certificate of Origin
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