- CB_86041101
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biological source
mouse skin
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
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This Item | CB_85111801 | 850512P | SML1486 |
|---|---|---|---|
| biological source mouse skin | biological source mouse embryo | biological source - | biological source - |
| growth mode Adherent | growth mode Adherent | growth mode - | growth mode - |
| karyotype Adherent | karyotype Adherent | karyotype - | karyotype - |
| morphology Adherent | morphology Adherent | morphology - | morphology - |
| products Adherent | products Adherent | products - | products - |
| receptors Adherent | receptors Adherent | receptors - | receptors - |
General description
Product has lost contact inhibition
Cell Line Origin
Mouse fibroblast
Cell Line Description
A mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.
Application
Virus studies: Moloney MuLV, retroviruses
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificate of Analysis
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Certificate of Origin
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