- CB_90020104
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biological source
mouse lung
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
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This Item | CB_86121112 | SML0991 | CB_87061101 |
|---|---|---|---|
| biological source mouse lung | biological source Porcine kidney | biological source - | biological source rat unknown/unspecified |
| growth mode Adherent | growth mode Adherent | growth mode - | growth mode Adherent |
| karyotype Adherent | karyotype Adherent | karyotype - | karyotype Adherent |
| morphology Adherent | morphology Adherent | morphology - | morphology Adherent |
| products Adherent | products Adherent | products - | products Adherent |
| receptors Adherent | receptors Adherent | receptors - | receptors Adherent |
Cell Line Origin
Mouse C57BL Lewis lung carcinoma
Cell Line Description
Derived from the lung of a C57BL mouse implanted with a primary Lewis Lung carcinoma. The cells can be used as a model for metastasis and for studying the effects of chemotherapeutic agents.
Application
Tumourigenicity, Metastasis and chemotherapeutic agent studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using mechanical technique (aspiration); 5% CO2; 37°C. Cells detach as aggregates and are therefore difficult to count.
Other Notes
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Certificate of Analysis
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Certificate of Origin
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