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biological source

mouse blood

growth mode



Not specified




Not specified


Not specified


cell culture | mammalian: suitable

shipped in

dry ice

storage temp.


Related Categories

Cell Line Origin

Mouse C57b1/6 T cell

Cell Line Description

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.


CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml.

Other Notes

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Certificate of Analysis

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Certificate of Origin

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