MilliporeSigma
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CGD1

Sigma-Aldrich

Cell Growth Determination Kit, MTT based

All Photos(1)
Synonym(s):
Mitochondrial activity assay
NACRES:
NA.75

packaging

pkg of 1 kit

technique(s)

UV/Vis spectroscopy: suitable
protein quantification: suitable

Amax

570 nm

application(s)

cell analysis
detection

detection method

colorimetric

shipped in

dry ice

storage temp.

−20°C

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Cell Growth Determination Kit, MTT based

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MTT Cell Growth Assay Kit

technique(s)

UV/Vis spectroscopy: suitable, protein quantification: suitable

technique(s)

-

technique(s)

tissue culture: suitable

technique(s)

activity assay: suitable, cell based assay: suitable

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

pkg of 1 kit

packaging

-

Amax

570 nm

Amax

-

Amax

-

Amax

-

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

cell analysis
detection

application(s)

-

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

-

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

wet ice

General description

Cell Growth Determination Kit (MTT based) is designed for the spectrophotometric measurement of cell growth as a function of mitochondrial activity in living cells. The MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) system is a simple, accurate, reproducible means of measuring the activity of living cells via mitochondrial dehydrogenase activity.

Application

Cell Growth Determination Kit, MTT based has been used:
  • to measure the cell viability of preosteoblastic cells
  • in MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay of normal human prostatic stromal and epithelial cell lines
  • to determine the cell viability of primary cervical epithelial cells

Packaging

Each kit includes 5 vials each containing 1 mL of 5 mg/ml MTT in RPMI (without phenol red) and 50 mL of MTT solvent (0.1 N HCl in anhydrous isopropanol). Volumes of kit components have been optimized for cultures grown in multi-well plates. MTT solutions is added at 10% of total culture volume, thus the number of tests per kit is dependent upon culture vessel and/or volume.

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12 °C - closed cup

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