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CS0008

Sigma-Aldrich

Enzymatic Protein Biotinylation Kit

for biotinylation of up to 20 mg target protein

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Quality Level

shipped in

dry ice

storage temp.

−70°C

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This Item
EDEGLYBTAGB2059
storage temp.

−70°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Biotinylated proteins are used in a variety of applications, such as binding assays, biopanning, and protein purification. Although chemical biotinylation methods are common, they present several challenges, including partial labeling and high batch to-batch variation, because of the random nature of these methods. In addition, the lack of control of the biotinylation site might affect the biotinylated protein′s activity. The Enzymatic Protein Biotinylation Kit allows direct and specific biotinylation using a specific biotin ligation enzyme. The kit contains all the components required for in vitro biotinylation of up to 20 mg recombinant target protein. Biotinylation is mediated by the enzyme BirA biotin ligase, which uniformly ligates biotin to lysine residues in the context of several tags, such as AviTag and Biotag. This kit was tested successfully under a wide range of conditions, including:
  • Temperatures ranging from 4 °C to 30 °C
  • pH from 6.0 to 9.0
  • NaCl concentrations from 50 to 500 mM.
The kit includes streptavidin, which can be used to assess the degree of biotinylation. The kit also includes a BLPR-tagged protein as a positive control.
Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs.

Legal Information

AviTag is a trademark of Avidity, LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 2


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