This product is a sterile-filtered M Quality Level 300 product. It is not molecular biology grade and does not have specifications such as RNase and DNase free. Therefore, this product cannot be considered RNase free for use in in situ hybridization.
D8537
Dulbecco′s Phosphate Buffered Saline
liquid, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Dulbecco’s Phosphate Buffered Saline
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Synonym(s):
DPBS
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100 ML
$17.60
500 ML
$32.90
1 L
$65.10
6 X 500 ML
$182.00
6 X 1 L
$346.00
24 X 500 ML
$657.00
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100 ML
$17.60
500 ML
$32.90
1 L
$65.10
6 X 500 ML
$182.00
6 X 1 L
$346.00
24 X 500 ML
$657.00
About This Item
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Product Name
Dulbecco′s Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: no (Dextro)
sodium pyruvate: no
shipped in
ambient
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This Item | 59321C | 59331C | 59300C |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 300 | Quality Level 400 | Quality Level 400 | Quality Level 500 |
| form liquid | form liquid | form liquid | form liquid |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| impurities endotoxin, tested | impurities - | impurities - | impurities - |
d-PBS, Dulbeccos PBS, Dulbeccos Buffer, Phosphate Buffered Saline, buffered saline Dulbeccos, balanced salt solution (BSS), bss for mammalian cells, | Isotonic saline, cell washing solution, cell suspension solution | Isotonic saline, cell washing solution, cell suspension solution | DPBS, Isotonic saline, cell washing solution, cell suspension solution |
General description
Dulbecco’s Phosphate Buffered Saline (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS buffer is used to irrigate, wash, and dilute mammalian cells while maintaining osmotic balance. It provides water and inorganic ions essential for normal cell metabolism. DPBS with calcium and magnesium facilitates cell binding and clumping. Dulbecco′s PBS without these ions can be used to wash and rinse suspended cells. Phosphate buffering maintains the pH in the physiological range.
Application
Dulbecco′s Phosphate Buffered Saline has been used:
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Quería saber si éste producto se puede considerar RNAsa free para realizar durante la hibridación in situ
1 answer-
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Bonjour, pourriez-vous m'indiquer le numéro de CAS de la solution de phosphate saline dulbecco sous forme liquide
1 answer-
This item is a multi-component product. A CAS number cannot be applied to this type of product. The Safety Data Sheet document includes the individual CAS numbers of those components that may be potentially hazardous.
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Hi, is this product GMP-grade or are GMP-garde material is used for the production of this?
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This product is not GMP-grade. Unfortunately. a GMP-grade version of this product is not available.
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What is the concentration of the product in Molarity?
1 answer-
This is a multi-component formulation. A 'molarity' is not reported for this type of product. See below for the list of components:
Product D8537 contains:
Potassium chloride: 0.2 g/l
Potassium phosphate monobasic: 0.2 g/l
Sodium chloride: 8 g/l
Sodium Phosphate dibasic: 1.15 g/lPlease see the link below for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/124/464/d8537for.pdfHelpful?
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What is the bottle thread type on the 1L bottle (D8537-1L)
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The product is packaged in a square PET bottle with an HDPE cap. The cap specifications are as follows:
Thread Type 38-430
Thread Diameter 37.3 mmHelpful?
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What is a Salt Toxicity Test?
1 answer-
The Cell Culture assay is called a Salt Toxicity Test (previously called Cell Culture Salt Testing). A more complete description is below:Cells are harvested and counted. They are resuspended in DPBS and incubated at 37°C, 5% CO2 for 1 hour. The cells are counted and %viability determined. The cells are then cultured for an additional 7 hours (total of 8 hours). The cells are counted again and the % viability determined. The viability at 1 hr has to be >95% of the original viability. The viability at 8 hrs must be >75% of the original viability.
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What is the pH of a solution of Product D8537, Dulbecco's Phosphate?
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The pH will be in the range of 7.1-7.5, according to the specification sheet.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product D8537, Dulbecco's Phosphate, a 1X or 10 X concentration?
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This is a 1X concentration. The 10X concentration is listed under product D1408.
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What is the formulation for Product D8537, Dulbecco's Phosphate?
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The formulation for 1 liter is 8 g Sodium Chloride, 0.2 g Potassium Phosphate, monobasic, 1.15 g Sodium Phosphate, dibasic, and 0.2 g Potassium Chloride.
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