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DUO92101

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Duolink® In Situ Red Starter Kit Mouse/Rabbit

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Synonym(s):
in situ Proximity Ligation Assay, Protein Protein Interaction Assay
NACRES:
NA.32

product line

Duolink®

Quality Level

technique(s)

proximity ligation assay: suitable

fluorescence

λex 594 nm; λem 624 nm (Texas Red®; Zeiss Filter set 31)

suitability

suitable for fluorescence

storage temp.

−20°C

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DUO92008DUO92013DUO92007
technique(s)

proximity ligation assay: suitable

technique(s)

proximity ligation assay: suitable

technique(s)

proximity ligation assay: suitable

technique(s)

proximity ligation assay: suitable

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

suitability

suitable for fluorescence

product line

Duolink®

product line

Duolink®

product line

Duolink®

product line

Duolink®

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Application

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® In Situ Red Starter Kit Mouse/Rabbit requires one primary antibody from mouse and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list
Duolink® in situ red starter kit has been used in proximity ligation assay.

Features and Benefits

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Legal Information

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

Kit Components Also Available Separately

Product No.
Description
SDS

  • DUO92002Duolink® In Situ PLA® Probe Anti-Rabbit PLUS, Affinity purified Donkey anti-Rabbit IgG (H+L)SDS

  • DUO92004Duolink® In Situ PLA® Probe Anti-Mouse MINUS, Affinity purified Donkey anti-Mouse IgG (H+L)SDS

  • DUO92008Duolink® In Situ Detection Reagents RedSDS

  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS

  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 2 - Met. Corr. 1 - Skin Sens. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3


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JNK associated leucine zipper protein functions as a docking platform for Polo like kinase 1 and regulation of the associating transcription factor Forkhead box protein K1.
Ramkumar P, et al.
The Journal of Biological Chemistry (2015)
Targeting CPT1A-mediated fatty acid oxidation sensitizes nasopharyngeal carcinoma to radiation therapy.
Tan Z, et al.
Theranostics, 8(9), 2329-2329 (2018)
New protein?protein interactions of mitochondrial connexin 43 in mouse heart.
Denuc A, et al.
Journal of Cellular and Molecular Medicine, 20(5), 794-803 (2016)
Ivan Duran et al.
Human molecular genetics, 24(7), 1918-1928 (2014-12-17)
Osteogenesis imperfecta (OI) is a genetic disorder that results in low bone mineral density and brittle bones. Most cases result from dominant mutations in the type I procollagen genes, but mutations in a growing number of genes have been identified
Roberto Di Maio et al.
Science translational medicine, 10(451) (2018-07-27)
Missense mutations in leucine-rich repeat kinase 2 (LRRK2) cause familial Parkinson's disease (PD). However, a potential role of wild-type LRRK2 in idiopathic PD (iPD) remains unclear. Here, we developed proximity ligation assays to assess Ser1292 phosphorylation of LRRK2 and, separately

Articles

Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.

Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Support information including tips and tricks, frequently asked questions, and basic troubleshooting.

Protocols

This page details the Duolink® In Situ Short Protocol for fluorescence detection

This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.

Related Content

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

Applications to detect, quantify and visualize protein-protein interactions, post-translational modifications and low expression protein detection using proximity ligation assay

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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