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F1804

ANTI-FLAG® M2 antibody, Mouse monoclonal

1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

ANTI-FLAG® M2 antibody, Mouse monoclonal

Synonym(s):

flag antibody, Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2

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50 μg
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$166.00
200 μg
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$559.00
1 mg
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$1,250.00
5 mg
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$2,020.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
M2, monoclonal
Application:
—
Citations:
9676

$166.00

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

purified by

affinity chromatography (affinity tag purification)

species reactivity

all

concentration

1.0-1.2 mg/mL

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

wet ice

storage temp.

−20°C

General description

The ANTI-FLAG M2 mouse, affinity purified monoclonal antibody binds to fusion proteins containing a FLAG peptide sequence.The antibody recognizes the FLAG peptide sequence at the N-terminus, Met-N-terminus,C-terminus, and internal sites of the fusion protein.

Method of purification - Affinity tag purification

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

For highly sensitive and specific detection of FLAG fusion proteins by immunoblotting, immunoprecipitation (IP) [1], immunohistochemisty, immunofluorescence and immunocyotchemistry. Optimized for single banded detection of FLAG fusion proteins in mammalian, plant, and bacterial expression systems.

Western Blotting and EIA

Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Binding site:N-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-C

Physical form

Contains 50% glycerol in phosphate buffered saline.

Preparation Note

Immediately before use, dilute in Tris buffered saline (TBS), pH 8.0, with 3% nonfat Milk

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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ANTI-FLAG® M2 antibody, Mouse monoclonal 1.0-1.2 mg/mL, clone M2, affinity isolated antibody, buffered aqueous solution (50% glycerol, 10 mM sodium phosphate, and 150 mM NaCl, pH 7.4)

Sigma-Aldrich

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ANTI-FLAG® M2 antibody, Mouse monoclonal

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ANTI-FLAG® M2 antibody, Mouse monoclonal clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Sigma-Aldrich

F3165

ANTI-FLAG® M2 antibody, Mouse monoclonal

ANTI-FLAG® BioM2 antibody, Mouse monoclonal clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Sigma-Aldrich

F9291

ANTI-FLAG® BioM2 antibody, Mouse monoclonal

biological source

mouse

biological source

-

biological source

mouse

biological source

mouse

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

species reactivity

all

species reactivity

-

species reactivity

all

species reactivity

all

antibody form

affinity isolated antibody

antibody form

-

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

biotin conjugate

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

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flash_point_f

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flash_point_c

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Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0000549952
0000549949
0000549947
0000427817
0000427814

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Articles

Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates

Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.

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Global Trade Item Number

SKUGTIN
F1804-50UG04061838261755
F1804-1MG04061838261748
F1804-200UG04061833612491
F1804-5MG04061838261762
3 Reviews This action will navigate to reviews.

Questions

1–5 of 5 Questions  

  1. Can this Antibody be used for Flow cytometry?

    1 answer

    1. While this antibody hasn't been validated for flow cytometry, independent laboratories have published its use for this application. For example:
      https://www.nature.com/articles/s42003-020-1072-4.pdf

      Helpful?

  2. Any protocol available for using the Anti-Flag M2 (F1804) in a ChIP-Seq assay? And, if the lysis buffer contains SDS and sodium deoxycholate at a concentration of 0.1%, will the presence of both denature or interfere with binding in the research process?

    1 answer

    1. Several papers have cited F1804 in ChIP & ChIPseq applications:
      https://www.nature.com/articles/s41467-023-40803-y.pdf
      https://www.nature.com/articles/s41467-023-41307-5.pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10541012&blobtype=pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10693086&blobtype=pdf
      https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC10863984&blobtype=pdf

      Anti-FLAG® M2 Magnetic Beads are not compatible with deoxycholate and SDS. Please refer to compatibility chart on the datasheet linked below for further information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/187/075/m8823bul-ms.pdf

      Helpful?

  3. Is this Ab suitable for ELISA?

    1 answer

    1. This antibody is compatible with enzyme immunoassay, which includes ELISA, the most widely used form of EIA.

      Helpful?

  4. Bonjour, il est indiqué que cet anticorps marche en immunohistochimie. Quel protocole d'antigen retrieval faut-il utiliser et quelle concentration d'anticorps sur coupe FFPE? Merci

    1 answer

    1. Please see the reference at the link below using this antibody at 1:50 dilution in IHC with antigen retrieval in citrate buffer for 5 minutes.
      https://www.nature.com/articles/s41467-019-12187-5.pdf

      Helpful?

  5. モノクローナル抗FLAG® M2抗体 マウス宿主抗体(F1804)を購入しました。WBで使用しますが、抗体の推奨の希釈率、希釈液を知りたいです。よろしくお願いいたします。

    1 answer

    1. An antibody concentration of 1 ug/mL or 1:1000 dilution is recommended for use in western blot. Please see the link below to review the product datasheet which includes the full protocol:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf

      Helpful?

Reviews

Active Filters

  1. Technion-Israel Institute of Technology
    • Review 1
    • Votes 22
    5 out of 5 stars.

    Perfection

    Perfect for WB and IF, very specific. Stable for many years.

    Helpful?

  2. 2 Ratings-Only Reviews