F4135

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

• Synonym(s): HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
• MDL number: MFCD00132239
• eCl@ss: 32160409
• NACRES: NA.71

Properties

• biological source: bovine fetus
• Quality Level: 500
• sterility: sterile-filtered
• product line: FBS Classic
• composition: Bovine IgG, ≤1 mg/mL ; Hemoglobin, ≤20 mg/dL
• origin: USA origin
• technique(s): cell culture | hybridoma: suitable; cell culture | insect: suitable; cell culture | mammalian: suitable
• impurities: ≤10 EU/mL endotoxin
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.

Application

FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:

• as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
• as a component of blocking medium for treating brainstem slices
• to improve attachment of dermal explants

Preparation Note

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

• Endotoxin and hemoglobin tested

• Tested for the presence of bacteria, virus, and mycoplasma
• Triple filtered with 0.1 micron membrane under aseptic conditions

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves