F8192

Fetal Bovine Serum

Australia origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for hybridoma

• Synonym(s): HI FBS, HI sera, HI serum, FBS, FCS, sera, serum
• MDL number: MFCD00132239

Properties

• biological source: bovine fetus
• Quality Level: 500
• sterility: sterile-filtered
• product line: FBS Classic
• composition: Bovine IgG, ≤1 mg/mL ; Hemoglobin, ≤20 mg/dL
• origin: Australia origin
• technique(s): cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• impurities: ≤10 EU/mL endotoxin
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Application

FBS derived from clotted blood is the most widely used undefined supplement in eucaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM); Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM); M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Preparation Note

Prepared by heating at 56°C for 30 minutes.

This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.

Analysis Note

• Endotoxin and hemoglobin tested

• Tested for the presence of bacteria, virus, and mycoplasma
• Triple filtered with 0.1 micron membrane under aseptic conditions

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable