G3272

Guanidine hydrochloride

for molecular biology, ≥99%

• Synonym(s): Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
• Linear Formula: NH₂C(=NH)NH₂ · HCl
• CAS Number: 50-01-1
• Molecular Weight: 95.53
• Beilstein: 3591990
• EC Number: 200-002-3
• MDL number: MFCD00013026
• PubChem Substance ID: 24895119
• NACRES: NA.31

Properties

• biological source: synthetic (organic)
• Quality Level: 400
• grade: for molecular biology
• Assay: ≥99%
• form: crystalline powder
• technique(s): RNA extraction: suitable
• impurities: ≤0.3% water (Karl Fischer)
• color: white
• pH: (25 °C, 4.6 - 6/573 g/L)
• mp: 180-185 °C (lit.)
• solubility: H₂O: 6 M, clear, colorless
• density: 1.3 g/cm³ (lit.)
• cation traces: Pb: ≤5 ppm
• UV absorption: λ: 260 nm A_max: ≤0.10; λ: 290 nm A_max: ≤0.05
• foreign activity: DNase, RNase, none detected
• SMILES string: Cl[H].NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N
• Gene Information: human ... KCNA1(3736) , KCNA10(3744) , KCNA2(3737) , KCNA3(3738) , KCNA4(3739) , KCNA5(3741) , KCNA6(3742) , KCNA7(3743) , KCNB1(3745) , KCNB2(9312) , KCNC1(3746) , KCNC2(3747) , KCNC3(3748) , KCNC4(3749) , KCND1(3750) , KCND2(3751) , KCND3(3752) , KCNF1(3754) , KCNG1(3755) , KCNG2(26251) , KCNG3(170850) , KCNG4(93107) , KCNH1(3756) , KCNH2(3757) , KCNH3(23416) , KCNH4(23415) , KCNH5(27133) , KCNH6(81033) , KCNH7(90134) , KCNH8(131096) , KCNQ1(3784) , KCNQ2(3785) , KCNQ3(3786) , KCNQ4(9132) , KCNQ5(56479) , KCNS1(3787) , KCNS2(3788) , KCNS3(3790) , KCNV1(27012) , KCNV2(169522)

Description

Application

Guanidine hydrochloride has been used:

• as a component of the extraction buffer for the extraction of proteoglycans
• in extraction during protein fractionation of ATDC5 cell lines
• as a chemical additive to study its effective absorbance spectra in structural analysis
• to incubate the device chip to release prostate-specific antigen and for regeneration of aptamer
• as a component of the 2,4-dinitrophenylhydrazine (DNPH) solution for detecting protein carbonyls by protein carbonylation study
• in RNA isolation to dissociate nucleoproteins and inhibit RNase

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GuHCl) is a small hydroscopic molecule. It plays a role in inhibiting heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) activity in vivo. It is a potent denaturant and inactivator of several enzymes and proteins. GuHCl can inactivate aminoacylase and papain. It is involved in unfolding and inactivation of alkaline phosphatase in Haliotis diversicolor. At higher concentrations, GuHCl is involved in virus inactivation such as Herpes simplex virus 1 (HSV-1).

Caution

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302 + H332 - H315 - H319
• Precautionary Statements: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves