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G9277

Sigma-Aldrich

Guanidine thiocyanate

for molecular biology, ≥99%

Synonym(s):
Guanidinium rhodanide, Guanidinium thiocyanate
Linear Formula:
NH2C(=NH)NH2 · HSCN
CAS Number:
Molecular Weight:
118.16
Beilstein:
3563461
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.31

biological source

synthetic (organic)

Quality Level

grade

for molecular biology

assay

≥99%

form

powder or crystals

technique(s)

RNA extraction: suitable

color

colorless to white,

mp

120-122 °C (lit.)

solubility

water: 100 mg/mL, clear, colorless

cation traces

Ba: ≤5 ppm
Fe: ≤5 ppm
Na: ≤0.5%
heavy metals (as Pb): ≤5 ppm

application(s)

agriculture

foreign activity

DNase, RNase, none detected

SMILES string

SC#N.NC(N)=N

InChI

1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H

InChI key

ZJYYHGLJYGJLLN-UHFFFAOYSA-N

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Application

Guanidine thiocyanate has been used:
  • for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation
  • as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
  • for DNA extraction from cerebrospinal fluid (CSF)

Chaotropic agent and strong denaturant; solubilizes cells.

Packaging

100, 250, 500 g in poly bottle

Biochem/physiol Actions

Guanidine thiocyanate is a denaturing agent and is used routinely in RNA isolation. It is used as a storage buffer for whole blood samples. Guanidine thiocyanate inactivates nucleases and is ideal for storing and freezing fecal samples for DNA studies. It is used in combination with phenol−chloroform in RNA extraction.

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

Storage Class Code

8B - Non-combustible, corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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