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H3375

Sigma-Aldrich

HEPES

≥99.5% (titration)

Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

Assay

≥99.5% (titration)

form

crystalline powder

technique(s)

cell culture | mammalian: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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HEPES ≥99.5% (titration)

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HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

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H4034

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HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99% (titration)

form

crystalline powder

form

crystalline powder

form

powder

form

crystalline

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

pH

5.0-6.5 (25 °C, 238 g/L)

pH

-

pH

5.0-6.5 (1 M in H2O)

pH

-

solubility

water: 500 mg/mL, clear, colorless

solubility

-

solubility

H2O: 1 M, clear, colorless

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
  • As a component of Hanks′ solution used in the degradation testing of Iron
  • As a component of HEPES medium in the transfection of cell culture with plasmids
  • Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
  • As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

Other Notes

Go to Redi-Dri Product Listing RDD002
Easily compare specifications for HEPES products with the HEPES specification table.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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