Histones are soluble in water at a concentration of 10 mg/ml or in 0.5 N HCl at the same concentration, yielding a clear or hazy solution depending on the fraction. Histones dissolved or suspended in water should remain stable for at least 6 months when frozen in single-use aliquots.
H5505
Histone from calf thymus
Type III-S
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About This Item
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biological source
bovine (calf) thymus
Quality Level
type
Type III-S
form
powder
concentration
≥60% protein (biuret)
technique(s)
activity assay: suitable
solubility
H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
storage temp.
2-8°C
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This Item | H4380 | H9250 | SRP2051 |
|---|---|---|---|
| biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine (calf) thymus | biological source bovine (calf) thymus |
| technique(s) activity assay: suitable | technique(s) - | technique(s) - | technique(s) membrane array: suitable |
| concentration ≥60% protein (biuret) | concentration - | concentration - | concentration 900 μg/mL |
| form powder | form powder | form lyophilized powder | form frozen liquid |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −70°C |
| solubility H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow | solubility - | solubility - | solubility - |
Application
Histone is suitable for use:
- as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein[1]
- in Cdk5 activity assay[2]
- in histone H1 kinase assay[3]
- as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity[4]
- as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells[5]
Biochem/physiol Actions
Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA.[6] Based on the fractions, the molar mass of histones varies between 11-21kDa.[7] Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich.[8] H1 acts as a link between "beads" (nu bodies) on the chromatin chain.[6]
Features and Benefits
Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.
Preparation Note
Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).
Other Notes
Characterized as mainly subgroup f1 by SDS-PAGE
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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P Balsas et al.
Journal of hematology & oncology, 10(1), 80-80 (2017-04-01)
Pharmacological inhibition of B cell receptor (BCR) signaling has recently emerged as an effective approach in a wide range of B lymphoid neoplasms. However, despite promising clinical activity of the first Bruton's kinase (Btk) and spleen tyrosine kinase (Syk) inhibitors
Amit K Dutta et al.
Journal of colloid and interface science, 324(1-2), 55-60 (2008-05-30)
The real-time changes in viscoelasticity of adsorbed poly(L-lysine) (PLL) and adsorbed histone (lysine rich fraction) due to cross-linking by glutaraldehyde and corresponding release of associated water were investigated using a quartz crystal microbalance with dissipation monitoring (QCM-D) and attenuated total
Mária Čížková et al.
Cells, 8(7) (2019-07-20)
DNA damage is a ubiquitous threat endangering DNA integrity in all living organisms. Responses to DNA damage include, among others, induction of DNA repair and blocking of cell cycle progression in order to prevent transmission of damaged DNA to daughter
Scott T
Concise encyclopedia of biochemistry, 265-265 (1988)
Anne Cecília Nascimento da Cruz et al.
Pharmaceuticals (Basel, Switzerland), 12(4) (2019-11-21)
Fourteen arylsemicarbazone derivatives were synthesized and evaluated in order to find agents with potential anticancer activity. Cytotoxic screening was performed against K562, HL-60, MOLT-4, HEp-2, NCI-H292, HT-29 and MCF-7 tumor cell lines. Compounds 3c and 4a were active against the
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What type of buffer or solution would be best for stabilizing histone proteins? Would ultrapure water (ddH₂O) be suitable for dissolution?
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