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H6147

Sigma-Aldrich

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

product line

BioXtra

Assay

≥99.5% (titration)

form

powder

technique(s)

cell culture | embryo: suitable

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5
7.5

solubility

water: 500 mg/mL, clear, colorless

absorption

≤0.05 at 290 in H2O at 33%

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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1 of 4

This Item
H7523H337554457
HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

H6147

HEPES

Premium Grade
HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

Premium Grade
HEPES ≥99.5% (titration)

Sigma-Aldrich

H3375

HEPES

-
HEPES BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

54457

HEPES

Premium Grade
assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

assay

≥99.5% (T)

form

powder

form

powder

form

crystalline powder

form

powder

technique(s)

cell culture | embryo: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

pH

5.0-6.5 (25 °C, 238 g/L)

pH

5.0-6.5 (1 M in H2O)

pH

5.0-6.5 (25 °C, 238 g/L)

pH

5.0-6.5 (25 °C, 1 M in H2O)

solubility

water: 500 mg/mL, clear, colorless

solubility

H2O: 1 M, clear, colorless

solubility

water: 500 mg/mL, clear, colorless

solubility

H2O: 1 M at 20 °C, clear, colorless

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Application

HEPES has been used:
  • As a constituent of modified Eagle′s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
  • As a component of modified Ham′s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
  • in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Other Notes

This product has been qualified for use in cell and mouse embryo culture.
Easily compare specifications for HEPES products with the HEPES specification table.

Physical properties

Useful pH range: 6.8 - 8.2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

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The effects of L-ergothioneine and L-ascorbic acid on the in vitro maturation (IVM) and embryonic development (IVC) of sheep oocytes.
Pancarci SM, et al.
Kafkas U?niversitesi veteriner faku?ltesi dergisi, 16, 757-763 (2010)
Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception.
Aslam I and Fishel S
Human Reproduction, 13(3), 634-638 (1998)
Viable offspring derived from cryopreserved haploid rabbit parthenotes.
Garci'a-Ximenez F and Escriba MJ
Theriogenology, 57(4), 1319-1325 (2002)
Lilianna Szyk-Warszyńska et al.
Polymers, 11(5) (2019-05-28)
Multilayer films containing α- and β-casein and polypeptides, poly-L-lysine (PLL), and poly-L-arginine (PLArg) were formed by the layer-by-layer technique and Fourier Transform InfraRed spectroscopy with Attenuated Total Reflection (FTIR-ATR) and FTIR/Grazing Angle analyzed their infrared spectra. We investigated the changes
Hydrogen ion buffers for biological research.
Good NE, et al.
Biochemistry, 5(2), 467-477 (1966)

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