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H7416

Sigma-Aldrich

Interleukin-6 human

IL-6, recombinant, expressed in HEK 293 cells, suitable for cell culture

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Synonym(s):
IL-6
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

≤2.0 ng/mL ED50

quality

endotoxin tested

mol wt

dimer 26-30 kDa (glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... IL6(3569)

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This Item
SCU0001SRP309611138600001
vibrant-m

H7416

Interleukin-6 human

vibrant-m

SCU0001

Interleukin-6 human

vibrant-m

SRP3096

Interleukin-6 human

vibrant-m

11138600001

Interleukin-6, human (hIL-6)

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in E. coli

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | hybridoma: suitable

biological source

human

biological source

human

biological source

human

biological source

human

Quality Level

100

Quality Level

200

Quality Level

300

Quality Level

100

form

lyophilized powder

form

lyophilized powder

form

lyophilized

form

solution

General description

The IL6 (interleukin-6) gene is mapped to human chromosome 7p15.3. All the members of IL6 family have membrane glycoprotein gp130 as a common receptor. IL6 (interleukin-6) induces B-cell differentiation and is a primary regulator of inflammation.

Application

Interleukin-6 human has been used to check its role in breast cancer metastasis. It has also been used for evaluating its role in D5HS tumor sphere formation.

Biochem/physiol Actions

IL6 regulates tumor proliferation and inhibits apoptosis. It contributes to the disease progression of coronary artery disease.
Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Preparation Note

Human IL-6, expressed in human 293 cells, is a glycosylated monomer with an apparent molecular mass of 26-30 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Customers Also Viewed

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The pro-and anti-inflammatory properties of the cytokine interleukin-6
Scheller J, et al.
Biochimica et Biophysica Acta, 1813(5), 878-888 (2011)
Generation of Prostate Tumor-initiating Cells is Associated with Elevation of Reactive Oxygen Species and IL6/STAT3 Signaling
Qu Y, et al.
Cancer Research (2013)
The-174 G> C interleukin-6 gene polymorphism is associated with angiographic progression of coronary artery disease over a 4-year period.
Toutouzas K, et al.
Hellenic Journal of Cardiology : HJC = Hell?nik? Kardiologik? epithe?r?s?, 58(1), 80-86 (2017)
Interplay between YB-1 and IL-6 promotes the metastatic phenotype in breast cancer cells
Castellana B, et al.
Oncotarget, 6(35), 38239-38239 (2015)
Donghong Chen et al.
Respiratory research, 22(1), 280-280 (2021-10-30)
IL-35 subunit EBI3 is up-regulated in pulmonary fibrosis tissues. In this study, we investigated the pathological role of EBI3 in pulmonary fibrosis and dissected the underlying molecular mechanism. Bleomycin-induced pulmonary fibrosis mouse model was established, and samples were performed gene

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