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H7666

Sigma-Aldrich

Interleukin-12 human

HumanKine®, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin, tested

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Synonym(s):
IL-12
MDL number:
NACRES:
NA.77

biological source

human

Quality Level

recombinant

expressed in HEK 293 cells

Assay

≥95% (SDS-PAGE)

form

lyophilized

potency

≤2 ng/mL EC50

mol wt

dimer 57 kDa (glycosylated)

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

−20°C

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This Item
H7291H9166H4666
Sigma-Aldrich

Sigma-Aldrich

H7666

Interleukin-12 human

Sigma-Aldrich

Sigma-Aldrich

H7291

Interleukin-4 human

Activin A human recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Sigma-Aldrich

H4666

Activin A human

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in HEK 293 cells

assay

≥95% (SDS-PAGE)

assay

>95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

form

lyophilized

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

potency

≤2 ng/mL EC50

potency

≤0.6 ng/mL EC50

potency

≤5 ng/mL EC50

potency

≤5 ng/mL EC50

mol wt

dimer 57 kDa (glycosylated)

mol wt

dimer 14-19 kDa (glycosylated)

mol wt

dimer 45 kDa (glycosylated)

mol wt

dimer 25 kDa (non-glycosylated)

Application

Interleukin-12 (IL-12) human has been used to study IL-12 mediated enhancement of IL-12Rβ2 (IL-12 receptor) expression.

Biochem/physiol Actions

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

lyophilized from a 0.2 μm filtered solution of PBS

Analysis Note

The specific activity was determined by the dose-dependent release of IFN-γ from the human NK92 cell line in presence of 20 ng/mL rIL-2.

Legal Information

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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T-bet upregulation and subsequent interleukin 12 stimulation are essential for induction of Th1 mediated immunopathology in Crohn's disease.
Matsuoka K et al.
Gut, 53, 1303-1303 (2004)

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