I0523

Interleukin-2 from mouse

IL-2, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): mIL-2, IL-2, T-cell growth factor
• MDL number: MFCD00164816
• NACRES: NA.77

Properties

• biological source: mouse
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤0.2 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: 17.2 kDa
• packaging: pkg of 5X20 μg; pkg of 20 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white
• UniProt accession no.: P04351
• storage temp.: −20°C
• Gene Information: mouse ... Il2(16183)

Description

General description

IL-2 (interleukin-2) codes for a pro-inflammatory cytokine. IL-2 is mostly expressed in T helper cells.

Application

Interleukin-2 from mouse has been used to induce sepsis in rat model. It has also been used in dendritic epidermal T cell culture.

Biochem/physiol Actions

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lymphokine promotes long-term growth of activated T cells and related cell types. IL-2 plays a role in the activation and proliferation of natural killer cells, induces γ-interferon and B cell growth factor secretion, and modulates the expression of the IL-2 receptor. IL-2 expression is known to reduce with age and results in influenza infection, neoplasms and lethal autoimmunity. IL-2 participates in T cell proliferation and differentiation and controls the homeostasis of T cell. As a part of immune response, IL-2 stimulates the action of T helper cell 1 and 2 along with endothelial white blood cells of skeletal muscles. IL-2 regulation is controlled by c-rel protein. IL-2 is a neurotrophic molecule and has special functions in cortical, hippocampal, septal, cerebellar and retinal neurons.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM sodium citrate, pH 4.0.

Analysis Note

Activity was determined by dose-dependent stimulation of murine CTLL-2 cells. ED₅₀ : ≤ 0.2 ng/ml (corresponds to a specific activity of 5 × 10⁶ units/mg)

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves