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I2276

Sigma-Aldrich

Interleukin-12 human

≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

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Synonym(s):
IL-12
MDL number:
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in baculovirus infected Sf21 cells

Assay

≥97% (SDS-PAGE and N-terminal analysis)

form

lyophilized powder

potency

0.01-0.05 ng/mL ED50

mol wt

glycoprotein 75 kDa

packaging

pkg of 5 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

Gene Information

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H7666I1771I1145
Sigma-Aldrich

Sigma-Aldrich

I2276

Interleukin-12 human

Sigma-Aldrich

Sigma-Aldrich

H7666

Interleukin-12 human

Interleukin-13 human ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sigma-Aldrich

I1771

Interleukin-13 human

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in HEK 293 cells

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf21 cells

assay

≥97% (SDS-PAGE and N-terminal analysis)

assay

≥95% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

assay

≥97% (SDS-PAGE)

form

lyophilized powder

form

lyophilized

form

lyophilized powder

form

lyophilized powder

mol wt

glycoprotein 75 kDa

mol wt

dimer 57 kDa (glycosylated)

mol wt

12 kDa

mol wt

45-50 kDa

packaging

pkg of 5 μg

packaging

pkg of 10 μg

packaging

pkg of 10 μg

packaging

pkg of 5 μg

Biochem/physiol Actions

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.25 mg bovine serum albumin.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Ruifen Sun et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 36(12), 9295-9301 (2015-06-25)
IL-12 is an antitumor cytokine with functions of inhibiting tumor growth, invasion, and metastasis, indicating that IL-12 is a promising candidate for cancer treatment. The aim of this study was to investigate the association of IL-12A rs568408, IL-12A rs2243115, and

Articles

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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