MilliporeSigma
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L1667

Sigma-Aldrich

Lysozyme human

recombinant, expressed in rice, ≥100,000 units/mg protein, lyophilized powder

Synonym(s):
Lysobac r Lysozyme, Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

recombinant

expressed in rice

Quality Level

assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥100,000 units/mg protein

mol wt

16.5 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... LYZ(4069)

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General description

Lysozyme is present in tears, saliva and human milk. The human lysozyme expressed in rice grains is an excellent supplemental source of this antimicrobial protein.[1] Lysozyme is mapped to human chromosome 12q15 and corresponds to a molecular weight of 16.5kDa.[2] It is a positively charged and a C-type lysozyme[3] with α and β structural domains.[4]

Application

Lysozyme human has been used:
  • in the interaction studies with polyphenon-60 by calorimetric and spectroscopic techniques[5]
  • in the drug release assay of dexamethasone (DEX) - carbon nanotube based delivery system[6]
  • in the surface plasmon resonance (SPR) for measuring its interaction with human angiotensin I-converting enzyme (ACE)[7]

Packaging

1, 10 g in glass bottle
Package size based on protein content

Biochem/physiol Actions

Lysozyme is an antibacterial enzyme, which acts on the peptidoglycan layer at the on the N-acetylgluosamine (NAG) and N-acetylmuramic acid linkage, resulting in the lysis of microbial cells.[1] It is a potential anti-inflammatory factor and also has anti-fungal and anti-viral functionality.[3] In inflammatory bowel disease, lysozyme is highly expressed in colonic tissue.[2] Point mutations in lysozyme is implicated in the pathogenesis of hereditary non-neuropathic renal amyloidosis.[4]

Features and Benefits

Lysobac is a breakthrough for bacterial cell lysis and can be used in diagnostic applications, bioprocessing and life science research. It is recombinant human lysozyme produced in an animal-free production system. Animal-free production eliminates the safety risk and inconsistent lot-to-lot performance of hen egg white lysozyme. Lysobac has significantly higher bioactivity than hen egg white lysozyme. One gram replaces 4 grams of hen egg white lysozyme. It also delivers gentle cell lysis, unlike mechanical lysis which can cause protein shear and reduce protein yields by up to 20%.
Advantages:
  • Improves Bacterial Fermentation Efficiency
  • Greater bacterial lysis activity per mg.
  • 1 gram of delivers more lysis activity than 4 grams of HEWL
  • Animal-free and consistent leading to improved regulatory efficiency for drug manufacturing
  • Improved recombinant protein yield compared to mechanical lysis
  • Reduces cost of goods

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25 °C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Legal Information

Lysobac is a trademark of InVitria

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

More Documents

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