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L2879

Sigma-Aldrich

Lysozyme chloride form from chicken egg white

Grade VI, ≥35,000 units/mg protein (E1%/282)

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Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number:
9066-59-5
Enzyme Commission number:
3.2.1.17 (BRENDA, IUBMB)
EC Number:
232-954-0
MDL number:
MFCD00131557
NACRES:
NA.54

biological source

chicken egg white

type

Grade VI

form

lyophilized powder

specific activity

≥35,000 units/mg protein (E1%/282)

mol wt

~14.3 kDa

composition

Protein, ≥80%

storage temp.

−20°C

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This Item
T2011T9253G1642
Sigma-Aldrich

Sigma-Aldrich

L2879

Lysozyme chloride form from chicken egg white

Trypsin inhibitor from chicken egg white Type III-O (free of ovoinhibitor)

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T2011

Trypsin inhibitor from chicken egg white

Trypsin inhibitor from chicken egg white Type II-O, Partially purified ovomucoid, containing ovoinhibitor

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T9253

Trypsin inhibitor from chicken egg white

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Sigma-Aldrich

G1642

sn-Glycerol-3-phosphocholine Phosphodiesterase from mold

form

lyophilized powder

form

powder

form

powder

form

lyophilized powder

specific activity

≥35,000 units/mg protein (E1%/282)

specific activity

-

specific activity

-

specific activity

≥5 units/mg protein

mol wt

~14.3 kDa

mol wt

14 kDa

mol wt

14 kDa

mol wt

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Enzyme which breaks down the cell walls of bacteria; used to prepare spheroplasts.

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Physical form

Lyophilized powder containing sodium chloride and sodium acetate

Preparation Note

3× Crystallized

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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A Sobrino-López et al.
Journal of dairy science, 91(5), 1759-1768 (2008-04-19)
High-intensity pulsed electric field (HIPEF) is a nonthermal treatment studied for its wide antimicrobial spectrum on liquid food, including milk and dairy products. Moreover, the antimicrobial effect of HIPEF may be enhanced by combining HIPEF with other treatments as hurdles.
R S Pappas et al.
Journal of analytical toxicology, 32(4), 281-291 (2008-04-24)
The extent to which smokeless tobacco endangers human health is an ongoing subject of debate. Studies have shown that smokeless tobacco products contain high levels of biologically available nicotine and tobacco-specific nitrosamines. Toxic metals in smokeless tobacco products have been
Andrea Weeks et al.
Eye & contact lens, 39(2), 179-185 (2013-02-16)
This study was designed to assess the duration of hyaluronic acid (HA) release from model contact lens materials when HA was physically incorporated into the hydrogel during synthesis and to assess the effects of the HA release on lysozyme sorption.
Masaki Honda et al.
Transplantation, 95(4), 551-558 (2013-02-21)
Neutrophils are considered responsible for the pathophysiologic changes during hepatic ischemia-reperfusion (I/R) injury; however, few studies have examined real-time intravital neutrophil recruitment. Here, we show a method for imaging the neutrophil recruitment in hepatic I/R injury using two-photon laser scanning
Agnieszka Zdybicka-Barabas et al.
Biochimica et biophysica acta, 1828(6), 1449-1456 (2013-02-20)
Insect immune response relies on the humoral and cellular mechanisms of innate immunity. The key factors are the antimicrobial polypeptides that act in concert against invading pathogens. Several such components, e.g. apolipophorin III (apoLp-III), lysozyme, and anionic peptide 2, are
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Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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