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L3790

Sigma-Aldrich

Lysozyme from chicken egg white

10 mg/mL

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Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.56

biological source

chicken egg white

Quality Level

form

liquid

mol wt

single-chain 14.3 kDa

concentration

≥400000 units/mL
10 mg/mL

technique(s)

cell based assay: suitable

suitability

suitable for cell lysis

UniProt accession no.

application(s)

cell analysis

shipped in

wet ice

storage temp.

−20°C

Gene Information

chicken ... LYZ(396218)

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This Item
L491962971L6876
Lysozyme from chicken egg white 10 mg/mL

L3790

Lysozyme from chicken egg white

Lysozyme from chicken egg white BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

L4919

Lysozyme from chicken egg white

Lysozyme from chicken egg white powder (crystalline), ~70000 U/mg

62971

Lysozyme from chicken egg white

Lysozyme from chicken egg white lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

L6876

Lysozyme from chicken egg white

form

liquid

form

lyophilized powder

form

powder (crystalline)

form

lyophilized powder

concentration

≥400000 units/mL, 10 mg/mL

concentration

-

concentration

-

concentration

-

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

UniProt accession no.

P00698

UniProt accession no.

-

UniProt accession no.

P00698

UniProt accession no.

P00698

General description

Lysosyme is an enzyme found in egg white that breaks down the cell walls in bacteria enhancing the efficiency of DNA and protein extraction.

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.
Suitable for the purification of both DNA and protein from bacteria.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Suitability

Tested for suitability with CelLytic B in protein extraction from bacteria.

Physical form

Ready to use solution:10 mg/mL lysozyme in 25 mM sodium acetate pH 4.5 with 50% glycerol.
Solution in 50% glycerol, 25 mM Sodium Acetate

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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