MilliporeSigma
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L4025

Sigma-Aldrich

Lyticase from Arthrobacter luteus

lyophilized powder, ≥200 units/mg solid

Synonym(s):
(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase
CAS Number:
MDL number:
NACRES:
NA.54

biological source

bacterial (Arthrobacter luteus)

Quality Level

form

lyophilized powder

specific activity

≥200 units/mg solid

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

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Application

Lyticase from Arthrobacter luteus has been used:
  • as a component in spheroplasting buffer to prepare yeast nuclear extracts[1]
  • for digestion in sample preparation for β-glucan enzymatic assay using yeast[1]
  • to lyse cells for RNA isolation[2]

Packaging

25000, 50000, 100000, 250000, 1000000 units in poly bottle

Biochem/physiol Actions

Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation.[3] Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities.[4] It is useful with yeast cells such as e.g. Candida,[5] Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Unit Definition

One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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