L4402

Lysostaphin from Staphylococcus staphylolyticus

BioUltra, ≥97% (SDS-PAGE), Protein 40-60 % by biuret, ≥2,000 units/mg protein

• Synonym(s): Glycyl-glycine Endopeptidase
• CAS Number: 9011-93-2
• Enzyme Commission number: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• NACRES: NA.54

Properties

• product line: BioUltra
• Quality Level: 300
• assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• specific activity: ≥2,000 units/mg protein
• mol wt: 25 kDa
• composition: Protein, 40-60% biuret
• antibiotic activity spectrum: Gram-positive bacteria
• Mode of action: cell wall synthesis | interferes
• storage temp.: −20°C

Description

General description

Chemical structure: peptide

Package size based on protein content

Packaging

0.5, 2, 5 mg in glass bottle

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37 °C in a 6.0 ml reaction mixture.

Physical form

Contains potassium phosphate buffer salts and sodium chloride

Preparation Note

Affinity purified

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves