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L4919

Sigma-Aldrich

Lysozyme from chicken egg white

BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

Synonym(s):
Mucopeptide N-acetylmuramoylhydrolase, Muramidase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:

Quality Level

grade

for molecular biology

product line

BioUltra

assay

≥98% (SDS-PAGE)

form

lyophilized powder

specific activity

≥40,000 units/mg protein

mol wt

single-chain 14.3 kDa

composition

Protein, ≥90%

storage temp.

−20°C

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General description

Lysozyme is abundantly found in animal and plant kingdoms. It is a natural food preservative and also found to be present in specific bacterial cell walls. Lysozyme is usually present in tears, milk, urine and saliva.

Application

Lysozyme from chicken egg white has been used:
  • in dielectric spectroscopy studies of dynamics of protein
  • as a control to measure the human lysozyme activity
  • as a supplement in soaking solution to treat lenses

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Packaging

500 mg in poly bottle
1, 5 g in poly bottle

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
Lysozymes participate in the defense mechanism. It has the ability to stimulate catalysis by bringing steric stress in the substrates.

Features and Benefits

  • Highly purified by repeated crystallization and dialysis
  • Each lot is use-tested for isolation of plasmid DNA from E. coli

Unit Definition

One unit will produce a ΔA450 of 0.001 per min at pH 6.24 at 25°C, using a suspension of Micrococcus lysodeikticus as substrate, in a 2.6 mL reaction mixture (1 cm light path).

Preparation Note

3× crystallized

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Certificate of Origin

Corneal cell adhesion to contact lens hydrogel materials enhanced via tear film protein deposition
Elkins CM, et al.
Testing, e105512-e105512 (2014)
Metabolic engineering of probiotic Saccharomyces boulardii
Liu JJ, et al.
Applied and Environmental Microbiology, 82(8), 2280-2287 (2016)
Natural Food Antimicrobial Systems (2000)
Protein dynamics in a broad frequency range: Dielectric spectroscopy studies
Nakanishi M and Sokolov AP
Journal of Non-Crystalline Solids, 407, 478-485 (2015)
M K Suleiman et al.
PloS one, 14(12), e0220679-e0220679 (2019-12-27)
Kuwait is a semi-arid region with soils that are relatively nitrogen-poor. Thus, biological nitrogen fixation is an important natural process in which N2-fixing bacteria (diazotrophs) convert atmospheric nitrogen into plant-usable forms such as ammonium and nitrate. Currently, there is limited

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Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

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