L7386

Lysostaphin from Staphylococcus staphylolyticus

lyophilized powder, Protein 50-70 % by biuret, ≥500 units/mg protein

• Synonym(s): Glycyl-glycine Endopeptidase
• CAS Number: 9011-93-2
• Enzyme Commission number: 3.4.24.75 (BRENDA, IUBMB)
• MDL number: MFCD00131556
• NACRES: NA.54

Properties

• biological source: microbial (Staphylococcus staphylotyticus)
• Quality Level: 300
• form: lyophilized powder
• specific activity: ≥500 units/mg protein
• mol wt: 25 kDa
• composition: Protein, 50-70% biuret
• packaging: pkg of (sold on basis of mg protein)
• solubility: water: 2.00-2.10 mg/mL, clear to slightly hazy, colorless to light yellow
• antibiotic activity spectrum: Gram-positive bacteria
• application(s): diagnostic assay manufacturing; diagnostic assay manufacturing
• Mode of action: cell wall synthesis | interferes
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Chemical structure: peptide

Packaging

1, 5, 15, 500 mg

Biochem/physiol Actions

Lysostaphin is a zinc endopeptidase with a molecular weight of approximately 25 kDa. Because lysostaphin cleaves the polyglycine cross-links in the peptidoglycan layer of the cell wall of Staphylococcus species it has been found useful for cell lysis and also as a potential anti-microbial therapeutic.
pH Optimum for activity: ~7.5

Unit Definition

One unit will reduce the turbidity (A₆₂₀) of a suspension of Staphylococcus aureus cells from 0.250 to 0.125 in 10 min at pH 7.5 at 37°C in a 6.0 ml reaction mixture.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P304 + P340 + P312 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves