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  • LY0100
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LY0100

Sigma-Aldrich

Lysozyme Activity Kit

sufficient for 100 tests

All Photos(1)
Synonym(s):
Lysozyme assay kit
NACRES:
NA.26

usage

sufficient for 100 tests

Quality Level

200

storage temp.

2-8°C

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Lysozyme Activity Kit sufficient for 100 tests

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Lysozyme Activity Kit

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usage

sufficient for 100 tests

usage

-

usage

-

usage

sufficient for 100 colorimetric or fluorometric tests

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

-

Quality Level

-

General description

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.

Application

Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme, lysozyme purified from chicken egg white and lysozyme from pilocarpine-stimulated saliva.

Biochem/physiol Actions

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.

Storage and Stability

The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.

Kit Components Also Available Separately

Product No.
Description
SDS
  • L6876Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg proteinSDS
  • Lysozyme Reaction Buffer
  • M3770Micrococcus lysodeikticus ATCC No. 4698, suitable for substrate for the assay of lysozyme, lyophilized cellsSDS

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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