All Photos(1)



Medium 199

With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture





cell culture | mammalian: suitable


sodium pyruvate: no
L-glutamine: yes
NaHCO3: no
Hanks’ salts (2% CO2): yes
phenol red: yes

shipped in


storage temp.


Looking for similar products? Visit Product Comparison Guide

Related Categories


Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.


Formulated to contain 10.6 grams of powder per liter of medium.


Supplement with 0.35 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Thomas H C Sit et al.
Nature, 586(7831), 776-778 (2020-05-15)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first detected in Wuhan in December 2019 and caused coronavirus disease 2019 (COVID-19)1,2. In 2003, the closely related SARS-CoV had been detected in domestic cats and a dog3. However, little is known about the
Song Wang et al.
Frontiers in physiology, 8, 1004-1004 (2017-12-23)
P311 was identified to markedly promote cutaneous wound healing by our group. Angiogenesis plays a key role in wound healing. In this study, we sought to define the role of P311 in skin wound angiogenesis. It was noted that P311
Johannes Dittmann et al.
Journal of the mechanical behavior of biomedical materials, 77, 764-775 (2017-08-12)
The present study focused on the material characterisation of porcine oocytes by experiments combined with an inverse finite-element method (iFEM) approach. In doing so, two different deformation states, compression and indentation, were realised to enable the validation of the numerical
Alberto Stracuzzi et al.
Biomechanics and modeling in mechanobiology, 20(2), 751-765 (2021-02-04)
Probing mechanical properties of cells has been identified as a means to infer information on their current state, e.g. with respect to diseases or differentiation. Oocytes have gained particular interest, since mechanical parameters are considered potential indicators of the success


Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.


Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service