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M2154

Sigma-Aldrich

Medium 199

With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

2.5-4 (25 °C, 209 g/L)

components

phenol red: yes
NaHCO3: yes
HEPES: no
L-glutamine: no
Earle’s salts (5% CO2): yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

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Application

Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Effects of a proinflammatory response on metabolic function of cultured, primary ruminal epithelial cells.
Kent-Dennis, et al.
Journal of Dairy Science, 104, 1002-1017 (2021)
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
Melisa Gomez-Velazquez et al.
PLoS genetics, 13(8), e1006985-e1006985 (2017-08-29)
Cardiac progenitors are specified early in development and progressively differentiate and mature into fully functional cardiomyocytes. This process is controlled by an extensively studied transcriptional program. However, the regulatory events coordinating the progression of such program from development to maturation
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Martyna Pakuła et al.
International journal of molecular sciences, 20(1) (2019-01-06)
The role of the epithelial-mesenchymal transition (EMT) in ovarian cancer cell progression is unquestioned. In this report, we describe that malignant ascites, fluid that accumulates in the peritoneal cavity in a large group of patients with ovarian cancer, stimulate EMT

Articles

Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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