M3440

Mutanolysin Assay Substrate

Name: Mutanolysin Assay Substrate
Brand: SIGMA
Price: 233 CAD

lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls

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$233.00

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.28

Key Documents

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Product Name

Mutanolysin Assay Substrate, (Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.)

Quality Level

200

form

(Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.)

packaging

vial of 10 assays

shipped in

wet ice

storage temp.

−20°C

Related Categories

Cell Lysis Buffers & Protein Extraction Reagents

Protein Biology

Protein Sample Prep

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1 of 4

This Item	M3770	A7472	M0508
Sigma-Aldrich M3440 Mutanolysin Assay Substrate	Sigma-Aldrich M3770 Micrococcus lysodeikticus ATCC No. 4698	Sigma-Aldrich A7472 Allophycocyanin	Sigma-Aldrich M0508 Micrococcus lysodeikticus ATCC No. 4698
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form (Lyophilized suspension of washed Enterococcus faecalis STF-3 (ATCC 12984) cell walls.)	form lyophilized cells	form suspension	form lyophilized cells
storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
shipped in wet ice	shipped in -	shipped in wet ice	shipped in -
packaging vial of 10 assays	packaging -	packaging -	packaging -

Application

The activity of mutanolysin can be determined by hydrolysis of the rehydrated cell suspension in a continuous spectrophotometric assay.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

0000461022

0000371875

0000285178

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Lipid II overproduction allows direct assay of transpeptidase inhibition by β-lactams.

Yuan Qiao et al.

Nature chemical biology, 13(7), 793-798 (2017-05-30)

Peptidoglycan is an essential crosslinked polymer that surrounds bacteria and protects them from osmotic lysis. β-lactam antibiotics target the final stages of peptidoglycan biosynthesis by inhibiting the transpeptidases that crosslink glycan strands to complete cell wall assembly. Characterization of transpeptidases

Characterization of lytic enzyme open reading frame 9 (ORF9) derived from Enterococcus faecalis bacteriophage phiEF24C.

Jumpei Uchiyama et al.

Applied and environmental microbiology, 77(2), 580-585 (2010-11-26)

In bacteriophage (phage) therapy against Gram-positive bacteria, such as Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis, members of a genus of SPO1-like viruses are typically employed because of their extreme virulence and broad host spectrum. Phage φEF24C, which is a

Protocols

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

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Mutanolysin Assay Substrate