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M4782

Sigma-Aldrich

Mutanolysin from Streptomyces globisporus ATCC 21553

0.2 μm filtered, lyophilized powder, ≥4000 units/mg protein (biuret)

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CAS Number:
MDL number:

Quality Level

sterility

0.2 μm filtered

form

lyophilized powder

specific activity

≥4000 units/mg protein (biuret)

mol wt

23 kDa

shipped in

wet ice

storage temp.

−20°C

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This Item
M9901SRE0007C9830
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

23 kDa

mol wt

23 kDa

mol wt

23 kDa

mol wt

-

sterility

0.2 μm filtered

sterility

-

sterility

-

sterility

-

General description

Mutanolysin from Streptomyces globisporus consists of two main lytic enzymes and may be a useful agent for dental caries control.

Application

Provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA.

Biochem/physiol Actions

Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the β-N-acetylmuramyl-(1→4)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus.

Unit Definition

One unit will produce a ΔA600 of 0.01 per minute at pH 6.0 at 37 °C in a 1 mL volume using a suspension of Streptococcus faecalis cell wall as substrate.

Physical form

Lyophilized powder containing Ficoll and sodium succinate buffer salts

Preparation Note

Prepared from M 9901

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Tom Vanhoutte et al.
FEMS microbiology ecology, 48(3), 437-446 (2004-06-01)
According to the current insights, the predominant bacterial community in human feces is considered to be stable and unique for each individual over a prolonged period of time. In this study, the temporal stability of both the predominant population and
A rapid and efficient method of lysis of Listeria and other gram-positive bacteria using mutanolysin
Fliss, I., et al.
Biotechniques, 11, 456-457 (1991)
Composition of gastrointestinal microbiota in association with treatment response in individuals with metastatic castrate resistant prostate cancer progressing on enzalutamide and initiating treatment with anti-PD-1 (pembrolizumab).
Peiffer, et al.
Neoplasia, 32, 100822-100822 (2022)
John M Pfeffer et al.
Journal of bacteriology, 188(3), 902-908 (2006-01-24)
The O acetylation of peptidoglycan occurs specifically at the C-6 hydroxyl group of muramoyl residues. Using a combination of high-performance liquid chromatography-based organic acid analysis and carbohydrate analysis by high-pH anion-exchange chromatography, we determined that strains of Entercoccus durans, E.
Carli B Jones et al.
Frontiers in genetics, 13, 799615-799615 (2022-04-19)
Short read 16 S rRNA amplicon sequencing is a common technique used in microbiome research. However, inaccuracies in estimated bacterial community composition can occur due to amplification bias of the targeted hypervariable region. A potential solution is to sequence and assess

Protocols

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