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Medium 199

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture




cell culture | mammalian: suitable


sodium pyruvate: no
L-glutamine: yes
Earle’s salts (5% CO2): yes
phenol red: yes
NaHCO3: no

shipped in


storage temp.


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Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.


Formulated to contain 9.5 grams of powder per liter of medium.


Supplement with 2.2 g/L sodium bicarbonate.

Storage Class Code

13 - Non Combustible Solids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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The advantage of using laser for assisted hatching in routine assisted reproductive technology (ART) practice is debatable. Recently, it has been shown that laser-manipulated mouse embryos had compromised genetic integrity. However, the impact of laser-assisted hatching (LAH) on the epigenetic
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In sepsis-induced inflammation, polymorphonuclear neutrophils (PMNs) contribute to vascular dysfunction. The serine proteases proteinase 3 (PR3) and human leukocyte elastase (HLE) are abundant in PMNs and are released upon degranulation. While HLE's role in inflammation-induced endothelial dysfunction is well studied
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Molecular oncology, 10(10), 1559-1574 (2016-10-13)
Valosin-containing protein (VCP) or p97, a member of AAA-ATPase protein family, has been associated with various cellular functions including endoplasmic reticulum-associated degradation (ERAD), Golgi membrane reassembly, autophagy, DNA repair, and cell division. Recent studies identified VCP and ubiquitin proteasome system
Annaïck Carles et al.
Cancer research, 80(17), 3480-3491 (2020-07-10)
The somatic missense point mutation c.402C>G (p.C134W) in the FOXL2 transcription factor is pathognomonic for adult-type granulosa cell tumors (AGCT) and a diagnostic marker for this tumor type. However, the molecular consequences of this mutation and its contribution to the


Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.


Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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Medium 199 Formulation

Medium 199 Formulation

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