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Medium 199

HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture


Quality Level






cell culture | mammalian: suitable


endotoxin, tested

pH range



NaHCO3: yes
Earle’s salts (5% CO2): yes
phenol red: yes
L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM

shipped in


storage temp.


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Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.


Supplement with 0.1 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Marten Michaelis et al.
Andrology, 8(5), 1419-1427 (2020-04-20)
Recently we described two outbred mouse lines which have been selected for high fertility. These mouse models doubled the number of offspring per litter. Although selected for a primarily female-trait of high fertility (increased litter size), we were interested whether
Marten Michaelis et al.
BMC genomics, 18(1), 889-889 (2017-11-22)
Many genes important for reproductive performance are shared by both sexes. However, fecundity indices are primarily based on female parameters such as litter size. We examined a fertility mouse line (FL2), which has a considerably increased number of offspring and
Uğur Şen et al.
Asian-Australasian journal of animal sciences, 31(6), 827-834 (2017-12-23)
The aim of this study was to compare the effects of 36.5°C and 38.5°C incubation temperatures on the maturation of bovine oocytes and developmental competence of embryos. In experiment 1, oocytes were maturated in bicarbonate-buffered TCM-199 for 22 hours in
Wenjia Li et al.
Cell death & disease, 11(7), 599-599 (2020-08-01)
Alpha-lipoic acid (α-LA), a well-known antioxidant, was proved to active ALDH2 in nitrate tolerance and diabetic animal model. However, the therapeutic advantage of α-LA for heart failure and related signaling pathway have not been explored. This study was designed to
Duo Xu et al.
Cell death & disease, 12(4), 406-406 (2021-04-17)
Escape from programmed cell death is a hallmark of cancer. In this study, we investigated the anti-apoptotic mechanisms and explored the therapeutic potential of BCL-2 homology domain-3 (BH3) mimetics in malignant pleural mesothelioma (MPM), a lethal thoracic malignancy with an


Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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Medium 199 Formulation

Medium 199 Formulation

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