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M7653

Sigma-Aldrich

Medium 199

With Hanks′ salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Cell Culture Medium, M199 Medium, Medium 199 Solution, TCM 199

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500 ML
$65.50
6 X 500 ML
$301.00

$65.50

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500 ML
$65.50
6 X 500 ML
$301.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

$65.50

Please contact Customer Service for Availability
Request a Bulk Order

Product Name

Medium 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
NaHCO3: yes
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
phenol red: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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This Item
M7528M0393M9163
Sigma-Aldrich

M7653

Medium 199

Medium 199 HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

M7528

Medium 199

Medium 199 With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

M0393

Medium 199

Sigma-Aldrich

M9163

Medium 199

form

liquid

form

liquid

form

powder

form

liquid

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
NaHCO3: yes
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
phenol red: yes
HEPES: no

components

L-glutamine: no
sodium pyruvate: no
HEPES: 25 mM
phenol red: yes
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes

components

phenol red: yes
HEPES: no
sodium pyruvate: no
Hanks’ salts (2% CO2): yes
L-glutamine: no
NaHCO3: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.
Modification of the original Medium 199.Earle′s salts are replaced with Hanks′ salts. Hanks′ salts use a lower concentration of bicarbonate and different concentrations and salt forms of the essential inorganic ions.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

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Description
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used together

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Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBL5859
RNBL2346
RNBK1499
RNBJ2933
RNBJ1757

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