M8662

Mineral oil

PCR Reagent

• Synonym(s): Mineral oil for PCR
• CAS Number: 8042-47-5
• EC Number: 232-455-8
• MDL number: MFCD00131611
• UNSPSC Code: 12181504
• NACRES: NA.52
• Technique(s): PCR: suitable

Properties

• form: liquid
• packaging: vial of 1.5 mL (Total volume 7.5 mL (5 vials))
• technique(s): PCR: suitable
• color: colorless
• refractive index: n20/D 1.467 (lit.)
• density: 0.84 g/mL at 25 °C (lit.)
• foreign activity: DNase, RNase, protease, none detected
• storage temp.: room temp
• InChI key: AEOVEGJBKQQFOP-DDVLFWKVSA-L

Description

General description

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.[1]

Application

Mineral oil has been used:

• for routine PCR amplifications
• to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification[2]
• to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform[3][4]

Features and Benefits

• Provided in a convenient 5 × 1.5 mL (1 vial) pack size
• Tested for the absence of DNase, RNase, and protease.
  

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): No data available
• Flash Point(C): No data available
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves