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Medium 199

10 ×, With Hanks′ salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture


Quality Level






10 ×


cell culture | mammalian: suitable


endotoxin, tested


L-glutamine: no
sodium pyruvate: no
NaHCO3: no
phenol red: yes
Hanks’ salts (2% CO2): yes

shipped in


storage temp.


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Originally developed as a completely defined media formulation for the culture of primary explants. These media have broad applicability, particularly for non-transformed cells and are widely used for vaccine production, primary pancreatic explants, and lens tissues.


Supplement with 0.1 g/L L-glutamine, 0.35 g/L sodium bicarbonate at 1×.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Wei Qi et al.
Journal of pharmaceutical sciences, 107(8), 2070-2078 (2018-05-02)
Stable formulations of inactivated polio vaccine (IPV) could reduce cold-chain requirements and increase distribution of the vaccine to developing countries. Recently, significant improvement in thermal stability of IPV vaccines has been achieved by including urea in lyophilized formulations. In the
Xiaofeng Yang et al.
Bioscience reports, 40(10) (2020-09-29)
Transplantion of bone marrow-derived endothelial progenitor cells (EPCs) may be a novel treatment for deep venous thrombosis (DVT). The present study probed into the role of microRNA (miR)-361-5p in EPCs and DVT recanalization. EPCs were isolated from male Sprague-Dawley (SD)
Sujoy Dutta et al.
Eukaryotic cell, 7(7), 1146-1157 (2008-05-20)
Inherent deficiencies of Leishmania in heme biosynthesis were genetically complemented for delta-aminolevulinate-inducible biosynthesis and accumulation of light-excitable uroporphyrin. The phototoxic flagellar immobilization and cytolysis phenotypes and porphyrin mobilization noted previously were further analyzed biochemically and cytologically to delineate the mechanism
Ya-Guang Liu et al.
Molecular human reproduction, 15(10), 665-673 (2009-06-10)
Transforming growth factor beta 1 (TGF-beta1) levels are increased in the peritoneal fluid of endometriosis patients, and endometrial cells express TGF-beta signaling components; however, little is known regarding the role of TGF-beta in endometriosis. Our objective was to examine the
M Colomiere et al.
British journal of cancer, 100(1), 134-144 (2008-12-18)
Epidermal growth factor receptor (EGFR) is overexpressed in ovarian carcinomas, with direct or indirect activation of EGFR able to trigger tumour growth. We demonstrate significant activation of both signal transducer and activator of transcription (STAT)3 and its upstream activator Janus


Medium 199 - M199 Formulations

When properly supplemented, Medium 199 (M199) has broad species applicability, particularly for cultivation of non-transformed cells. It is widely used in virology, vaccine production and in vitro cultivation of primary explants of mouse pancreatic epithelial and rat lens tissues.

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Medium 199 Formulation

Medium 199 Formulation

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